Effect of the conductivity of the petri dish placed on the electrode on the surface layer of the spores in the PBII method
Shoya Umemoto, Koji Kakugawa, Kenta Nosaki, Takumi, Noda Takeshi Tanaka, Katia Vutova
Plasma-based ion implantation (PBII) method is a technique that allows uniform ion implantation to a sample by applying a negative voltage to the model to draw and accelerate ions in the ion sheath existing around the piece. We are researching to apply the PBII method as a new pasteurization technology. This study investigated how the different conductivity of Petri dishes used for sample processing affects the cell surface layer of Geobacillus stearothermophilus spores. The PBII treatment conditions were as follows: gas pressure: 5 Pa, treatment time: 10 min, gas type: nitrogen, applied voltage: -3 kV to -5 kV, RF: 60 VA, petri dish material: glass or stainless steel. After PBII treatment, the spore surface was observed using a scanning electron microscope. When spores were observed by scanning electron microscopy, it was found that the spore surface condition was different between the glass petri dish and the stainless steel petri dish. The sterilization effectiveness and ion density calculations at -3 kV showed significantly higher with the stainless steel petri dish. On the other hand, when the applied voltage was -5 kV, they were no longer quite different. It is suggested that this difference is due to differences in conductivity resulting from the material of the petri dish. Therefore, it was found that it is better to use a Petri dish having good conductivity to improve the sterilization effect using a lower applied voltage condition.
Cite this article as:
Kakugawa K., Umemoto S., Noda T., Nosaki K., Tanaka T., Vutova K. Effect of the conductivity of the petri dish placed on the electrode on the surface layer of the spores in the PBII method. Electrotechnica & Electronica (Е+Е), Vol. 57 (3-4), 2022, pp.41-45, ISSN: 0861-4717 (Print), 2603-5421 (Online)