The sterilization effect of plasma-based ion implantation on prokaryotic microorganisms
Shoya Umemoto, Koji Kakugawa, Kenta Nosaki, Takumi, Noda Takeshi Tanaka, Katia Vutova
The Plasma-based ion implantation (PBII) method is capable of low-temperature and short-time sterilization. In this study, we investigated the sterilization effect of PBII treatment on Gram-positive and Gram-negative bacteria’s vegetative cells. First, we examined the cell surface of Escherichia coli to clarify the effect of the different gas types on PBII. As a result of morphological change observed by scanning electron microscope, the surface of the cell on E. coli scraped and had many holes under the following conditions: O₂ atmosphere, 500 Hz frequency, 50 μs delay time,10 μs pulse width, −4 kV pulse voltage, 48 VA RF power, and a 10 min exposure time. It clarified that oxygen gas highly damaged vegetative cells more than the nitrogen gas treatment and the untreated. Second, we investigated PBII treatment’s effect on Gram-positive and Gram-negative bacteria. The sterilization effect of E. coli and Staphylococcus aureus were obtained 6.1 D and 6.9 D, respectively, under the O₂ atmosphere and −4 kV pulse voltage. Regardless of the differences in the cell wall structure, oxygen gas was able to sterilize prokaryotic vegetative cells effectively.
Cite this article as:
Umemoto S., Kakugawa K., Nosaki K., Noda T., Tanaka T, Vutova K. The sterilization effect of plasma-based ion implantation on prokaryotic microorganisms. Electrotechnica & Electronica (Е+Е), Vol. 57 (3-4), 2022, pp.46-50, ISSN: 0861-4717 (Print), 2603-5421 (Online)